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Extraction, Isolation and Characterization of Isopropyl Isothiocyanate from Seeds powder of Drypetes roxburghii Wall

Chandra Kala

Present study deals with the isolation and characterization of the isothiocyanate from the seed powder of Drypetes roxburghii. The seeds were dried and powdered. Extraction of the seed powder was performed under reflex using 800 ml of a hot mixture of light petroleum ether and ethanol (1:1 ratio) as solvent for 8hrs. The solvent ratio of light petroleum ether and ethanol (9:1) are used for removing the fat from the marc of the first extraction. Purification of the extract was performed by paper chromatography analytical technique with n-Butanol: Ethanol: Water (4:1:4) as solvent system. The Rf value of the the isolated compound was found to be 0.58. Isolated compound was confirmed by chemical test, characterization instrumental analytical technique like UV- spectrometer, FT-IR, 13C-NMR, and LC-MS. The IR absorption spectrum shows the 2875.47- 2983.74 cm-1 which indicate CH3 asymmetric vibrations stretching and 2730.18 cm-1 NCS asymmetric vibrations stretching, CN stretching. Experimented 1H NMR and 13C NMR confirmed the number of protons and carbons and their position. Structure elucidation of the isolated compound was done by Mass spectrometer analysis. The peak and M+1 of CH3CHNCSCH3 and CH3CHNCS was found to be (100.17 & 101.17) and (85.14 & 86.14) respectively.