抽象的な

Time Restricted Feeding are Associated with Changes in Human Gut Microbiota Related to Nutrients Intake

Falak Zeba

 Objectives: Time restricted feeding (TRF) is a dietary therapeutic remedy for the prevention and treatment of metabolic diseases. Gut microbiota may contribute to influence the host metabolism and nutritional status of the individuals. Given the significance of TRF and gut microbiota in metabolic diseases, this study was conducted to explore the association between TRF and gut microbiota in healthy individuals, which is not clearly elucidated. Methods: Thirty healthy males (age=18-30 years) were divided in to two groups (TRF, n=15 and Non-TRF, n=15). TRF group was instructed, to not use any food for 16 hours per day. Two days food dairy was used for dietary data collection. Stool samples were collected from both groups after 25 days of TRF or NonTRF. Gut microbiota profile was analyzed and quantified by using 16S rRNA gene sequencing. Results: Cluster analysis revealed that Prevotlla_9, Faecalibacterium and Dialister were the most abundant species in TRF, while Prevotell_7, Alloprevotella and Prevotella_2 were less abundant in Non-TRF group. At genus level, gut microbiota of TRF group was significantly changed from Non-TRF. Moreover, bar plot analysis revealed that Bacteroidetes was the most abundant phylum in TRF group followed by Firmicutes. Heatmap correlation showed that PUFA and vitamin D were positively correlated with Firmicutes, while iodine, vitamin E, magnesium and carbohydrates intakes were negative correlated with microbial richness.